Phospho-cdc25C (Ser216) Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3096R-100uL |
| Product Name | Phospho-cdc25C (Ser216) Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is found at Ser173 in Isoform 3 of this protein. |
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Ser216 |
| Modification Site | Ser216 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 995 |
| Swiss Prot | P30307 |
| Subcellular location | Nucleus |
| Synonyms | CDC25; PPP1R60; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C; CDC25C |
| Background | Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-cdc25C (Ser216) Polyclonal Antibody (bs-3096R) validated for, and at what dilution?
bs-3096R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3096R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-cdc25C (Ser216) Polyclonal Antibody (bs-3096R)?
KLH conjugated synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Ser216