phospho-eIF4EBP1 (Ser64) Polyclonal Antibody

$Antigen: bs-3018P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-3018P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

$Lane 1: mouse kidney lysates Lane 2: mouse embryo lysates probed with Anti phospho-eIF4EBP1\/4EBP1(Ser64) Polyclonal Antibody, Unconjugated (bs-3018R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 13kD. Observed band size: 13kD$

Lane 1: mouse kidney lysates Lane 2: mouse embryo lysates probed with Anti phospho-eIF4EBP1\/4EBP1(Ser64) Polyclonal Antibody, Unconjugated (bs-3018R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 13kD. Observed band size: 13kD

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-eIF4EBP1(Ser64)Polyclonal Antibody, Unconjugated (bs-3018R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-3018R
Product Name	phospho-eIF4EBP1 (Ser64) Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from mouse 4EBP1 around the phosphorylation site of Ser65
Modification Site	Ser65
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	13685
Swiss Prot	Q60876
Subcellular location	Cytoplasm
Synonyms	4e-bp1; PHAS-I; AA959816; Eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; Eif4ebp1
Background	Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200