C3a Receptor Polyclonal Antibody

293T lysates probed with C3a Receptor Polyclonal Antibody, Unconjugated (bs-2955R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

A549 lysates probed with C3a Receptor Polyclonal Antibody, Unconjugated (bs-2955R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Lane 1: Rat Spleen tissue lysates; Lane 2: Human A549 cell lysates; Lane 3: Human HL60 cell lysates; Lane 4: Human THP-1 cell lysates probed with C3a Receptor Polyclonal Antibody, Unconjugated (bs-2955R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Rat Spleen tissue lysates; Lane 2: Human A549 cell lysates; Lane 3: Human HL60 cell lysates; Lane 4: Human THP-1 cell lysates probed with C3a Receptor Polyclonal Antibody, Unconjugated (bs-2955R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Horse
Rabbit

Overview
Catalog #	bs-2955R
Product Name	C3a Receptor Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human C3a Receptor/C3aR
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	719
Swiss Prot	Q16581
Subcellular location	Cell membrane
Synonyms	AZ3B; C3AR; HNFAG09; C3a anaphylatoxin chemotactic receptor; C3a-R; C3AR1; C3R1
Background	Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200