IRS-1(Ser307) Polyclonal Antibody

Applications

  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
  • Goat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-2736R
Product Name IRS-1(Ser307) Polyclonal Antibody
Applications ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity This phosphorylation site is homologous to Ser302 in Mouse and Rat.
Reactivity Human, Mouse, Rat, Goat
Predicted Reactivity Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of Ser307 [TE(p-S)IT]
Modification Site Ser307
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3667
Swiss Prot P35568
Subcellular location Cell membrane
Synonyms HIRS-1; Insulin receptor substrate 1; IRS-1; IRS1
Background May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
Application Dilution
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200