MCT4/SLC16A3 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-SLC16A3 Polyclonal Antibody, Unconjugated(bs-2698R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$L1 rat kidney lysates, L2 rat brain lysates probed with Anti- SLC16A3\/MCT4 Polyclonal Antibody, Unconjugated (bs-2698R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 51kD$

L1 rat kidney lysates, L2 rat brain lysates probed with Anti- SLC16A3\/MCT4 Polyclonal Antibody, Unconjugated (bs-2698R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 51kD

Formalin-fixed and paraffin embedded rat brain labeled with Anti-MCT4 Polyclonal Antibody, Unconjugated (bs-2698R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat stomach labeled with Anti-MCT4 Polyclonal Antibody, Unconjugated (bs-2698R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MCT4 Polyclonal Antibody, Unconjugated (bs-2698R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MCT4 Polyclonal Antibody, Unconjugated (bs-2698R) at 1:400 overnight at 4\u00b0C, DAB staining.

Lane 1: Mouse Muscle lysates probed with MCT4 Polyclonal Antibody, Unconjugated (bs-2698R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Cow
Horse

Overview
Catalog #	bs-2698R
Product Name	MCT4/SLC16A3 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Cow, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MCT4
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9123
Swiss Prot	O15427
Subcellular location	Cell membrane
Synonyms	MCT3; MCT4; MCT 3; MCT 4; MCT-3; MCT-4; Monocarboxylate transporter 4; Solute carrier family 16 member 3; SLC16A3
Background	Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200