NRSF/REST Polyclonal Antibody

$Formalin-fixed and paraffin embedded human gastric tissue labeled with Anti NRSF\/REST Polyclonal Antibody,Unconjugated (bs-2590R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded human gastric tissue labeled with Anti NRSF\/REST Polyclonal Antibody,Unconjugated (bs-2590R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Formalin-fixed and paraffin embedded mouse brain labeled with Anti-NRSF\/REST Polyclonal Antibody, Unconjugated (bs-2590R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-NRSF\/REST Polyclonal Antibody, Unconjugated (bs-2590R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Raji lysates probed with NRSF Polyclonal Antibody, Unconjugated (bs-2590R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Lane 1: Hela cell lysates; Lane 2: Hela KO NRSF cell lysates probed with NRSF Polyclonal Antibody, Unconjugated (bs-2590R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: human hela cell lysates; Lane 2: bs-2590R IP in human hela cell lysates; Lane 3: native rabbit IgG IP in human hela cell lysates probed with NRSF\/REST Polyclonal Antibody, Unconjugated (bs-2590R) at 1:150 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: human hela cell lysates; Lane 2: bs-2590R IP in human hela cell lysates; Lane 3: native rabbit IgG IP in human hela cell lysates probed with NRSF\/REST Polyclonal Antibody, Unconjugated (bs-2590R) at 1:150 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-2590R
Product Name	NRSF/REST Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	KO-Validated
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NRSF
Immunogen Range	401-450/1097
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5978
Swiss Prot	Q13127
Subcellular location	Nucleus
Synonyms	XBR; NRSF; RE1-silencing transcription factor; Neural-restrictive silencer factor; X2 box repressor; REST
Background	Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200