ACCN2 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-ASIC1\/BNaC2\/ASIC1A Polyclonal Antibody, Unconjugated (bs-2586R) at 1:200, followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-ASIC1\/BNaC2\/ASIC1A Polyclonal Antibody, Unconjugated (bs-2586R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HL-60 cell lysates; Lane 4: Human U87MG cell lysates probed with ACCN2 Polyclonal Antibody, Unconjugated (bs-2586R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HL-60 cell lysates; Lane 4: Human U87MG cell lysates probed with ACCN2 Polyclonal Antibody, Unconjugated (bs-2586R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Horse
Chicken

Overview
Catalog #	bs-2586R
Product Name	ACCN2 Polyclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ASIC1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	41
Subcellular location	Extracellular
Synonyms	Acid-sensing ion channel 1; ASICs1; Acid sensing ion channel 1; Acid sensing ion channel 1a protein; Acid sensing ion channel; AI843610; Amiloride sensitive cation channel 2 neuronal; ASIC; ASIC1; ASIC1A; B530003N02Rik; BNAC2; Brain sodium channel 2; Cation channel amiloride sensitive neuronal 2; hBNaC2.
Background	Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.
Application Dilution
WB	1:500-2000