eIF4EBP1 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-2559R-100uL |
| Product Name | eIF4EBP1 Polyclonal Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
|
| Specificity | 60-70% sequence similarity to 4EBP2 |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human 4E-BP1 |
| Immunogen Range | 95-118/118 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 1978 |
| Synonyms | e4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E BP1; 4EBP1; BP 1; BP1; e4E binding protein 1; e4E-binding protein 1; Eukaryotic translation initiation factor 4E binding protein 1; MGC4316; PHAS I; PHASI; PHAS-I; PHAS; 4E-BP1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; Phosphorylated heat and acid stable protein regulated by insulin 1; 4EBP1_HUMAN. |
| Background | This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-P | 1:100-500 |
| IHC-F | |
| IF | |
Frequently asked questions
Which applications is eIF4EBP1 Polyclonal Antibody (bs-2559R) validated for, and at what dilution?
bs-2559R is validated for WB (1:500-2000); IHC-P (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eIF4EBP1 Polyclonal Antibody (bs-2559R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-2559R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise eIF4EBP1 Polyclonal Antibody (bs-2559R)?
KLH conjugated synthetic peptide derived from human 4E-BP1 Immunogen range: 95-118/118.