Get 20% off all conjugated antibody purchases with code "CON-ANTIBODY2025"! Offer valid on direct orders only. Available for US customers and internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

DMP1 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse bone); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (DMP1) Polyclonal Antibody, Unconjugated (bs-25502R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse bone); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (DMP1) Polyclonal Antibody, Unconjugated (bs-25502R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Cow
  • Sheep
  • Horse
Overview
Catalog # bs-25502r-100ul
Product Name DMP1 Polyclonal Antibody
Applications WB, IHC-P, IHC-F, IF
Reactivity Mouse
Predicted Reactivity Human, Rat, Cow, Sheep, Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DMP1
Immunogen Range 441-513/513
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 758
Swiss Prot Q13316
Subcellular location Extracellular, Cytoplasm, Secreted, Cell membrane
Synonyms ARHP; ARHR; AV020965; Dentin matrix acidic phosphoprotein 1; Dentin matrix protein 1; DENTMAT; DMP 1; Dmp; MGC130441; PP; Serine rich acidic phosphoprotein; DMP1_HUMAN.
Background DMP-1 is a member of the small integrin ligand N-linked glycoprotein family. It is important for the mineralization of bone and dentin. DMP-1 is expressed in bone, tooth and hypertrophic cartilage. It is synthesized by preosteoblasts and contains a large number of acidic domains. DMP-1 localizes to the nucleus of undifferentiated osteoblasts where it functions as a transcriptional regulator for osteoblast-specific gene activation and induces osteoblast differentiation. During osteoblast maturation, DMP-1 undergoes a conformational change and becomes phosphorylated by casein kinase II in response to an influx of calcium ions to the nucleus. DMP-1 is then exported to the extracellular matrix (ECM) where it regulates the nucleation of hydroxyapatite and the formation of calcified tissue. DMP-1 is proteolytically processed into N- and C-terminal fragments in the ECM of bone and dentin. The protein has also been identified in bone as a high molecular weight proteoglycan comprised of the N-terminal DMP-1 fragment and chondroitin sulfate.The loss of DMP-1 can result in hypomineralized bone.
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:100-500
IHC-F IHC-F=1:100-500
IF IF=1:100-500

Search our catalog of hundreds of thousands of products