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MyoD1/Myf3 Polyclonal Antibody

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IHC-F

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
  • Zebrafish
Overview
Catalog # bs-2442R-100uL
Product Name MyoD1/Myf3 Polyclonal Antibody
Applications
WB ELISA FCM IHC-P IHC-F
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse MyoD1
Immunogen Range 51-150/318
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 17927
Swiss Prot P10085
Subcellular location Nucleus
Synonyms MYF3; MyoD; Myod-1; bHLHc1; AI53393; Myoblast determination protein 1; Myod1
Background Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is MyoD1/Myf3 Polyclonal Antibody (bs-2442R) validated for, and at what dilution?

bs-2442R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MyoD1/Myf3 Polyclonal Antibody (bs-2442R) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish.

How should bs-2442R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MyoD1/Myf3 Polyclonal Antibody (bs-2442R)?

KLH conjugated synthetic peptide derived from mouse MyoD1 Immunogen range: 51-150/318.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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