UBE2Q1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UBE2Q1 Polyclonal Antibody, Unconjugated (bs-24324R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UBE2Q1 Polyclonal Antibody, Unconjugated (bs-24324R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UBE2Q1 Polyclonal Antibody, Unconjugated (bs-24324R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UBE2Q1 Polyclonal Antibody, Unconjugated (bs-24324R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UBE2Q1 Polyclonal Antibody, Unconjugated (bs-24324R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UBE2Q1 Polyclonal Antibody, Unconjugated (bs-24324R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UBE2Q1 Polyclonal Antibody, Unconjugated (bs-24324R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UBE2Q1 Polyclonal Antibody, Unconjugated (bs-24324R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P

Reactivity

Mouse
Rat

Overview
Catalog #	bs-24324R
Product Name	UBE2Q1 Polyclonal Antibody
Applications	IHC-P
Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human UBE2Q1:171-270/422
Immunogen Range	171-270/422
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	55585
Swiss Prot	Q7Z7E8
Subcellular location	Cytoplasm, Nucleus
Synonyms	Galactosyl transferase associated protein; GTAP; NICE 5; NICE5; PRO3094; Protein NICE 5; Protein NICE-5; UB2Q1_HUMAN; UBE2Q; Ube2q1; Ubiquitin carrier protein Q1; Ubiquitin conjugating enzyme E2 Q1; Ubiquitin conjugating enzyme E2Q (putative) 1; Ubiquitin conjugating enzyme E2Q 1; Ubiquitin conjugating enzyme E2Q family member 1; Ubiquitin protein ligase Q1; Ubiquitin-conjugating enzyme E2 Q1; Ubiquitin-protein ligase Q1.
Background	Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (UBE1s), ubiquitin-conjugating enzymes (UBE2s), and ubiquitin-protein ligases (UBE3s). When ubiquitin is activated by a UBE1, it is transferred to the cysteine residue on a UBE2. UBE2 then binds a UBE3, which transfers the ubiquitin from the UBE2 cysteine to a lysine residue on the target protein. Ubiquitin-conjugating enzyme E2 Q1 (UBE2Q1), also known as ubiquitin-protein ligase Q1 or ubiquitin carrier protein Q1, is an 422 amino acid protein belonging to the ubiquitin-conjugating enzyme (UBE2) family. Two named isoforms of UBE2Q1 exist as a result of alternative splicing events. Function : Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). Similarity : Belongs to the ubiquitin-conjugating enzyme family.
Application Dilution
IHC-P	1:200-400

Applications

Reactivity

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