SMO Polyclonal Antibody

$Lane 1: Mouse Eye lysates; Lane 2: NIH\/3T3 cell lysates probed with Smoothened Polyclonal Antibody, Unconjugated (bs-23988R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Eye lysates; Lane 2: NIH\/3T3 cell lysates probed with Smoothened Polyclonal Antibody, Unconjugated (bs-23988R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Recombinant human Smoothened protein, His probed with Smoothened Polyclonal Antibody, Unconjugated (bs-23988R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Recombinant human Smoothened protein, His probed with Smoothened Polyclonal Antibody, Unconjugated (bs-23988R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Horse
Rabbit

Overview
Catalog #	bs-23988R
Product Name	SMO Polyclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Smoothened
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	6608
Swiss Prot	Q99835
Synonyms	Gx; Gx protein; SMO; SMOH; Smoothened homolog (Drosophila); Smoothened homolog; Smoothened homolog precursor (SMO) (Gx protein); SMO_HUMAN.
Background	The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010]
Application Dilution
WB	1:300-5000