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Phospho-PERK (Thr980) Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Phospho-PERK ) Polyclonal Antibody, Unconjugated (bs-23340R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PERK ) Polyclonal Antibody, Unconjugated (bs-23340R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Rabbit
Overview
Catalog # bs-23340r-100ul
Product Name Phospho-PERK (Thr980) Polyclonal Antibody
Applications IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF
Reactivity Human, Mouse
Predicted Reactivity Rat, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthesised phosphopeptide derived from human PERK around the phosphorylation site of Thr980
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 13666
Swiss Prot Q9Z2B5
Synonyms p-PERK(Thr980); PERK(Phospho Thr980); PERK(Phospho T980); mo(Thr980)/hu (Thr982)/rat (Thr974); HRI; HsPEK; Pancreatic eIF2-alpha kinase; PEK; PRKR like endoplasmic reticulum kinase; WRS; DKFZp781H1925; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation factor 2 alpha kinase 3; Heme regulated EIF2 alpha kinase.
Application Dilution
IHC-P =1:100-500
IHC-F IHC-F=1:100-500
IF IF=1:100-500
Flow-Cyt Flow-Cyt=1ug/Test
ICC/IF ICC/IF=1:100

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