PKC epsilon Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-PKC epsilon Polyclonal Antibody, Unconjugated (bs-2329R) followed by conjugation to the secondary antibody

Formalin-fixed and paraffin embedded rat Testis tissue labeled with Anti-PKC epsilon Polyclonal Antibody, Unconjugated(bs-2329R) 1:300 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-PKC epsilon Polyclonal Antibody, Unconjugated(bs-2329R) 1:300 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded Human kidney tissue labeled with Anti-PKC epsilon Polyclonal Antibody, Unconjugated(bs-2329R) 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with PKC epsilon Polyclonal Antibody, Unconjugated (bs-2329R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with PKC epsilon Polyclonal Antibody, Unconjugated (bs-2329R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Dog
Cow
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-2329R
Product Name	PKC epsilon Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PKCE (AGC-kinase C-terminal)
Immunogen Range	631-737/737
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5581
Swiss Prot	Q02156
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	PKCE; nPKC-epsilon; Protein kinase C epsilon type; PRKCE
Background	Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500

Applications

Reactivity

Predicted Reactivity

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