HSL/LIPE Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HSL\/LIPE Polyclonal Antibody, Unconjugated (bs-22905R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HSL\/LIPE Polyclonal Antibody, Unconjugated (bs-22905R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Predicted Reactivity

Human
Mouse
Cow
Sheep
Pig

Overview
Catalog #	bs-22905R
Product Name	HSL/LIPE Polyclonal Antibody
Applications	IHC-P IHC-F IF
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Cow, Sheep, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human HSL/LIPE
Immunogen Range	441-540/1076
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3991
Swiss Prot	Q05469
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Hormone sensitive lipase; hormone-sensitive lipase; Hormone sensitive lipase testicular isoform; HSL; LHS; Lipase hormone sensitive; LIPE; Hormone-sensitive lipase; LIPS_HUMAN.
Background	In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Application Dilution
IHC-P	1:200-400
IHC-F
IF