phospho-p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2210R-100uL |
| Product Name | phospho-p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF
FLOW-CYT
ICC/IF
|
| Reactivity | Human, Mouse, Rat, Chicken, Goat |
| Predicted Reactivity | Dog, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human p38 MAPK around the phosphorylation site of(Thr180/Tyr182) |
| Modification Site | Thr180 + Tyr182 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1432 |
| Swiss Prot | Q16539 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA; Mitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Stress-activated protein kinase 2a; MAPK14 |
| Background | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | FCM1:20-100 |
| IHC-F | IHC-P1:200-400 |
| IF | ICC1:50-200 |
| Flow-Cyt | |
| ICC/IF | |
Frequently asked questions
Which applications is phospho-p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody (bs-2210R) validated for, and at what dilution?
bs-2210R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody (bs-2210R) react with?
Tested reactivity: Human, Mouse, Rat, Chicken, Goat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Pig, Horse, Rabbit.
How should bs-2210R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody (bs-2210R)?
KLH conjugated synthetic phosphopeptide derived from human p38 MAPK around the phosphorylation site of(Thr180/Tyr182)