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EGFP Polyclonal Antibody

Formalin-fixed and paraffin embedded rat liver labeled with Anti-EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:300 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded rat liver labeled with Anti-EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:300 followed by conjugation to the secondary antibody
Transfected HBX cells probed with Anti-EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Transfected HBX cells probed with Anti-EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli lysates(Cat#: bs-33009P) probed with EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli lysates(Cat#: bs-33009P) probed with EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA
  • IHC-P
  • IF(IHC-P)

Reactivity

  • Human
  • Rat
  • Tags
Overview
Catalog # bs-2194R
Product Name EGFP Polyclonal Antibody
Applications WB, ELISA, IHC-P, IF(IHC-P)
Specificity This antibody will work best with EGFP that has a similar amino acid make-up as that of Aequorea Victoria.
Reactivity Human, Rat, Tags
Specifications
Conjugation Unconjugated
Host Rabbit
Source Purified EGFP synthetic protein homologous to EGFP naturally occurring in Aequorea victoria
Immunogen Range 1-239/239
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms Enhanced Green Fluorescent Protein.
Background The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

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