PTN Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-21928R-Cy3-100uL |
| Product Name | PTN Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PTN |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5764 |
| Swiss Prot | P21246 |
| Subcellular location | Secreted |
| Synonyms | pleiotrophin; HARP; HBBM; HBGAM; HBGF8; HBNF; HBNF1; Heparin Binding Growth Factor 8; Heparin binding neurite outgrowth promoting factor 1; heparin-binding growth associated molecule; NEGF1; Neurite Growth Promoting Factor 1; OSF-1; osteoblast-specific factor; PTN; PTN_HUMAN. |
| Background | Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PTN Polyclonal Antibody, Cy3 Conjugated (bs-21928R-Cy3) validated for, and at what dilution?
bs-21928R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PTN Polyclonal Antibody, Cy3 Conjugated (bs-21928R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Horse, Rabbit.
How should bs-21928R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PTN Polyclonal Antibody, Cy3 Conjugated (bs-21928R-Cy3)?
KLH conjugated synthetic peptide derived from human PTN
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-21928R-Cy3)?
Cy3 on bs-21928R-Cy3: excitation / emission 512,550nm/570,615nm.