PARP1 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti PARP (N-Terminus) Polyclonal Antibody, Unconjugated(bs-2138R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI$

Formalin-fixed and paraffin embedded rat brain labeled with Anti PARP (N-Terminus) Polyclonal Antibody, Unconjugated(bs-2138R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI

Lane 1: Rat Brain; Probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:1000 in 4˚C.

Human HeLa lysates probed with Rabbit Anti-PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Human Raji lysates probed with Rabbit Anti-PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

$Lane 1: Hela Cells; Lane 2: Raji Cells; Lane 3: HepG2 Cells; Lane 4: K562 Cells; 30ug loaded into the lane; Probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:20000 for 60 minutes at 37\u00b0C. Lower band is the cleaved PARP1 form.$

Lane 1: Hela Cells; Lane 2: Raji Cells; Lane 3: HepG2 Cells; Lane 4: K562 Cells; 30ug loaded into the lane; Probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:20000 for 60 minutes at 37\u00b0C. Lower band is the cleaved PARP1 form.

$HL-60 cells were fixed with 70% methanol (Overnight at 4\u2103) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103. The cells were incubated in 1XPBS\/2%BSA\/10% goat serum for 15 min at room temperature to block non-specific protein-protein interactions. Next, the cells were probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:500 (0.2ug\/1x10^6 Cells) for 30 minutes at room temperature, followed by a Goat anti-rabbit IgG-PE (bs-0295G-PE) secondary antibody for 40 mins at room temperature. Acquisition of 20,000 events was performed. Primary Antibody staining (green) is compared to unstained cells (dark blue), secondary only (light blue) and isotype control (bs-0295P; orange).$

HL-60 cells were fixed with 70% methanol (Overnight at 4\u2103) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103. The cells were incubated in 1XPBS\/2%BSA\/10% goat serum for 15 min at room temperature to block non-specific protein-protein interactions. Next, the cells were probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:500 (0.2ug\/1x10^6 Cells) for 30 minutes at room temperature, followed by a Goat anti-rabbit IgG-PE (bs-0295G-PE) secondary antibody for 40 mins at room temperature. Acquisition of 20,000 events was performed. Primary Antibody staining (green) is compared to unstained cells (dark blue), secondary only (light blue) and isotype control (bs-0295P; orange).

Lane 1: Human 293T cell lysates; Lane 2: Human HL-60 cell lysates; Lane 3: Human K562 cell lysates; Lane 4: Human SH-SY5Y cell lysates; Lane 5: Human U251 cell lysates; Lane 6: Human Hela cell lysates probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bs-2138R
Product Name	PARP1 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PARP1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	142
Swiss Prot	P09874
Subcellular location	Nucleus
Synonyms	PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; Poly [ADP-ribose] polymerase 1; ADP-ribosyltransferase diphtheria toxin-like 1; NAD(+) ADP-ribosyltransferase 1; Poly[ADP-ribose] synthase 1; PARP1
Background	Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200