Cyp2-j3 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse heart labeled with Anti-Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse heart labeled with Anti-Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Lane 1: rat liver lysates Lane 2: rat brain lysates probed with Anti Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 55kD. Observed band size: 55kD.

Lane 1: rat liver lysates Lane 2: rat brain lysates probed with Anti Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 55kD. Observed band size: 55kD.

Lane 1: Liver lysates; Lane 2: Brain lysates probed with Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Lane 1: Liver lysates; Lane 2: Brain lysates probed with Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Overview
Catalog #	bs-2123R
Product Name	Cyp2-j3 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Cyp2-j3
Immunogen Range	401-502/502
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1573
Swiss Prot	P51589
Subcellular location	Cytoplasm, Cell membrane
Synonyms	CPJ2; Cytochrome P450 2J2; Arachidonic acid epoxygenase; CYPIIJ2; CYP2J2
Background	This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Powered by Bioz See more details on Bioz