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ZEB2 Polyclonal Antibody

Lane 1: Human Hela lysates; Lane 2: Human 293T lysates probed with SIP1 Polyclonal Antibody, Unconjugated (bs-20485R) at 1:300 dilution and 4ûC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37ûC.
Lane 1: Human Hela lysates; Lane 2: Human 293T lysates probed with SIP1 Polyclonal Antibody, Unconjugated (bs-20485R) at 1:300 dilution and 4ûC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37ûC.
Paraformaldehyde-fixed, paraffin-embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ZEB2 Polyclonal Antibody, Unconjugated (bs-20485R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin-embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ZEB2 Polyclonal Antibody, Unconjugated (bs-20485R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-20485R
Product Name ZEB2 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SIP1
Immunogen Range 721-820/1214
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
Storage Condition Store at -20¡C for 12 months.
Target
Gene ID 9839
Swiss Prot O60315
Synonyms Smad Interacting Protein 1; SIP 1; SIP1; SIP-1; Smad-interacting protein 1; SMADIP 1; SMADIP1; ZEB 2; ZEB2; ZEB2_HUMAN; Zfhx1b; ZFHX1B protein; Zfx1b; Zinc finger E box binding protein 2; Zinc finger E-box-binding homeobox 2; Zinc finger homeobox 1b; zinc finger homeobox protein 1; Zinc finger homeobox protein 1b.
Background Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(ICC) 1:50-200

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