IL-10 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin-embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes, DAB and counterstaining.$

Paraformaldehyde-fixed, paraffin-embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes, DAB and counterstaining.

$Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

$Lane 1: Rat Blood Plasma; Lane 2: Rat Blood Plasma; Probed with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Rat Blood Plasma; Lane 2: Rat Blood Plasma; Probed with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Lane 1: Mouse Raw264.7 Cells; Probed with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Raw264.7 Cells; Probed with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
ELISA
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-20373R
Product Name	IL-10 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human IL-10
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3586
Swiss Prot	P22301
Subcellular location	Secreted
Synonyms	CSIF; TGIF; GVHDS; IL-10; IL10A; Interleukin-10; Cytokine synthesis inhibitory factor; IL10
Background	Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. IL-10 Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400