IL-10 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-20373R-100uL |
| Product Name | IL-10 Polyclonal Antibody |
| Applications |
WB
ELISA
FCM
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IL-10 |
| Immunogen Range | 51-100/178 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3586 |
| Swiss Prot | P22301 |
| Subcellular location | Secreted |
| Synonyms | CSIF; TGIF; GVHDS; IL-10; IL10A; Interleukin-10; Cytokine synthesis inhibitory factor; IL10 |
| Background | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. IL-10 Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is IL-10 Polyclonal Antibody (bs-20373R) validated for, and at what dilution?
bs-20373R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IL-10 Polyclonal Antibody (bs-20373R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-20373R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IL-10 Polyclonal Antibody (bs-20373R)?
KLH conjugated synthetic peptide derived from human IL-10 Immunogen range: 51-100/178.