DLAT Polyclonal Antibody

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Heart tissue lysates; Lane 3: Mouse Muscle tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Muscle tissue lysates; Lane 6: Human PANC-1 cell lysates probed with PDCE2 Polyclonal Antibody, Unconjugated (bs-19695R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Heart tissue lysates; Lane 3: Mouse Muscle tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Muscle tissue lysates; Lane 6: Human PANC-1 cell lysates probed with PDCE2 Polyclonal Antibody, Unconjugated (bs-19695R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat
Zebrafish

Predicted Reactivity

Dog
Cow
Sheep
Pig
Horse
Cat
Rabbit
GuineaPig

Overview
Catalog #	bs-19695R
Product Name	DLAT Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human, Mouse, Rat, Zebrafish
Predicted Reactivity	Dog, Cow, Sheep, Pig, Horse, Cat, Rabbit, GuineaPig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PDCE2
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	1737
Swiss Prot	P10515
Synonyms	Pyruvate Dehydrogenase E2; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; anti DLAT; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Dihydrolipoamide; Dihydrolipoamide S Acetyltransferase; Dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex); Dihydrolipoamide S-Acetyltransferase; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial; DLAT; DLTA; E2; E2 component of pyruvate dehydrogenase complex; EC 2.3.1.12; M2 antigen complex 70 kDa subunit; M2 Antigen Complex 70kD Subunit; mitochondrial; ODP2_HUMAN; PBC; PDC E2; PDC-E2; PDCE2; Pyruvate dehydrogenase complex component E2; Pyruvate dehydrogenase complex E2 subunit; S acetyltransferase component of pyruvate dehydrogenase complex.
Background	The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF