GRM4 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-mGluR4 Polyclonal Antibody, Unconjugated (bs-1894R) at 1:200 followed by conjugation to the secondary antibody

$Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti GRM4\/mGluR4 Polyclonal Antibody, Unconjugated (bs-1894R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 102kD. Observed band size: 102kD.$

Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti GRM4\/mGluR4 Polyclonal Antibody, Unconjugated (bs-1894R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 102kD. Observed band size: 102kD.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Pig
Rabbit

Overview
Catalog #	bs-1894R
Product Name	GRM4 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	This antibody detects Metabotropic Glutamate Receptor 4. It may also detect Metabotropic Glutamate Receptor 6 due to sequence similarity.
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Pig, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Metabotropic Glutamate Receptor 4
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2914
Swiss Prot	Q14833
Subcellular location	Cell membrane
Synonyms	mGlu4; GPRC1D; MGLUR4; Metabotropic glutamate receptor 4; GRM4
Background	G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200