25% Off Flow Cytometry Antibodies - Use Code: "EggyIggy2024" - Valid 3/29-4/5/24 for U.S. customers only

LEF-1 Polyclonal Antibody

Applications

  • WB
  • ELISA
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Chicken
  • Rabbit
Overview
Catalog # bs-1843R
Product Name LEF-1 Polyclonal Antibody
Applications WB, ELISA, IHC-P
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Chicken, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human LEF-1
Immunogen Range 331-399/399
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 51176
Swiss Prot Q9UJU2
Subcellular location Nucleus
Synonyms LEF-1; TCF1; TCF7L3; TCF1ALPHA; Lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF1-alpha; LEF1
Background Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP3. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400