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PAN-CYP (3A4, 3A7, 3A5, and 3A9) Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-CYP3A4 Polyclonal Antibody, Unconjugated (bs-1742R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-CYP3A4 Polyclonal Antibody, Unconjugated (bs-1742R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-CYP3A4 Polyclonal Antibody, Unconjugated (bs-1742R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-CYP3A4 Polyclonal Antibody, Unconjugated (bs-1742R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse brain labeled with Anti-CYP3A4 Polyclonal Antibody, Unconjugated (bs-1742R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated (bs-0295G-FITC) used at 1:200 dilution for 40 minutes
Formalin-fixed and paraffin embedded mouse brain labeled with Anti-CYP3A4 Polyclonal Antibody, Unconjugated (bs-1742R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated (bs-0295G-FITC) used at 1:200 dilution for 40 minutes
Lane 1: rat liver lysates Lane 2: human colon carcinoma lysates probed with Anti CYP3A4 Polyclonal Antibody, Unconjugated (bs-1742R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 55kD. Observed band size: 55kD.\\n
Lane 1: rat liver lysates Lane 2: human colon carcinoma lysates probed with Anti CYP3A4 Polyclonal Antibody, Unconjugated (bs-1742R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 55kD. Observed band size: 55kD.\\n
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CYP3A4) polyclonal Antibody, Unconjugated (bs-1742R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CYP3A4) polyclonal Antibody, Unconjugated (bs-1742R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

  • WB
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)
  • ICC

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Rabbit
  • Guinea Pig
Overview
Catalog # bs-1742R
Product Name PAN-CYP (3A4, 3A7, 3A5, and 3A9) Polyclonal Antibody
Applications WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Specificity Will detect CYP 3A4, 3A7, 3A5, and 3A9
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Rabbit, Guinea Pig
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CYP3A4
Immunogen Range 18-120/503
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1576
Subcellular location Cytoplasm, Cell membrane
Synonyms Cytochrome p450 3A4; CP33; CP34; CYP3A; CYP3A3; CYP3A4; CYPIIIA4; CYPIIIA3; Cytochrome P450 family 3 subfamily A polypeptide 4; Cytochrome P450 subfamily IIIA polypeptide 4; Glucocorticoid inducible P450; HLP; MGC126680; NF 25; NF25; P450 III steroid inducible; P450 PCN1; P450C3; P450PCN1; Quinine 3 monooxygenase; Taurochenodeoxycholate 6 alpha hydroxylase; CP3A4_HUMAN; 1,8-cineole 2-exo-monooxygenase; Albendazole monooxygenase; Albendazole sulfoxidase; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nedipine oxidase; Quinine 3-monooxygenase; Taurochenodeoxycholate 6-alpha-hydroxylase; CYP3A4; CYP3A7; CYP3A5; CYP3A9
Background Cytochromes P450 are a group of heme thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8 oxidation, omeprazole sulphoxidation, midazolam 1 hydroxylation and midazolam 4 hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

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