PAN-CYP (3A4, 3A7, 3A5, and 3A9) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1742r-100uL |
| Product Name | PAN-CYP (3A4, 3A7, 3A5, and 3A9) Polyclonal Antibody |
| Applications |
WB
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | Will detect CYP 3A4, 3A7, 3A5, and 3A9 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Horse, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CYP3A4 |
| Immunogen Range | 18-120/503 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1576 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Cytochrome p450 3A4; CP33; CP34; CYP3A; CYP3A3; CYP3A4; CYPIIIA4; CYPIIIA3; Cytochrome P450 family 3 subfamily A polypeptide 4; Cytochrome P450 subfamily IIIA polypeptide 4; Glucocorticoid inducible P450; HLP; MGC126680; NF 25; NF25; P450 III steroid inducible; P450 PCN1; P450C3; P450PCN1; Quinine 3 monooxygenase; Taurochenodeoxycholate 6 alpha hydroxylase; CP3A4_HUMAN; 1,8-cineole 2-exo-monooxygenase; Albendazole monooxygenase; Albendazole sulfoxidase; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nedipine oxidase; Quinine 3-monooxygenase; Taurochenodeoxycholate 6-alpha-hydroxylase; CYP3A4; CYP3A7; CYP3A5; CYP3A9 |
| Background | Cytochromes P450 are a group of heme thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8 oxidation, omeprazole sulphoxidation, midazolam 1 hydroxylation and midazolam 4 hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PAN-CYP (3A4, 3A7, 3A5, and 3A9) Polyclonal Antibody (bs-1742R) validated for, and at what dilution?
bs-1742R is validated for WB (1:300-5000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PAN-CYP (3A4, 3A7, 3A5, and 3A9) Polyclonal Antibody (bs-1742R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Horse, Chicken.
How should bs-1742R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PAN-CYP (3A4, 3A7, 3A5, and 3A9) Polyclonal Antibody (bs-1742R)?
KLH conjugated synthetic peptide derived from human CYP3A4 Immunogen range: 18-120/503.