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CX3CR1 Polyclonal Antibody

Lane 1: rat brain lysates Lane 2: mouse heart lysates probed with Anti CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 39kD. Observed band size: 39kD.
Lane 1: rat brain lysates Lane 2: mouse heart lysates probed with Anti CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 39kD. Observed band size: 39kD.
Mouse splenocytes probed with Rabbit Anti-CX3CR1 Polyclonal Antibody, FITC Conjugated (bs-1728R-FITC) (green) at 1:100 for 40 minutes compared to control cells (blue).\\n
Mouse splenocytes probed with Rabbit Anti-CX3CR1 Polyclonal Antibody, FITC Conjugated (bs-1728R-FITC) (green) at 1:100 for 40 minutes compared to control cells (blue).\\n
Lane 1: Mouse Heart lysates; Lane 2: Rat Heart lysates probed with CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Heart lysates; Lane 2: Rat Heart lysates probed with CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Lung lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Mouse Spleen cell lysates probed with CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Lung lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Mouse Spleen cell lysates probed with CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Raw264.7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CX3CR1 Polyclonal Antibody(bs-1728R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Raw264.7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CX3CR1 Polyclonal Antibody(bs-1728R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CX3CR1) Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CX3CR1) Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Spinal cord tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Spinal cord tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Spinal cord tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Spinal cord tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IHC-F

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Rabbit
Overview
Catalog # bs-1728R-100uL
Product Name CX3CR1 Polyclonal Antibody
Applications
WB ELISA FCM IHC-P IHC-F
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CX3CR1
Immunogen Range 151-250/355
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1524
Swiss Prot P49238
Subcellular location Cell membrane
Synonyms V28; CCRL1; GPR13; CMKDR1; GPRV28; CMKBRL1; CX3C chemokine receptor 1; C-X3-C CKR-1; CX3CR1; Beta chemokine receptor-like 1; CMK-BRL-1; CMK-BRL1; Fractalkine receptor; G-protein coupled receptor 13
Background Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is CX3CR1 Polyclonal Antibody (bs-1728R) validated for, and at what dilution?

bs-1728R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CX3CR1 Polyclonal Antibody (bs-1728R) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Rabbit.

How should bs-1728R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CX3CR1 Polyclonal Antibody (bs-1728R)?

KLH conjugated synthetic peptide derived from human CX3CR1 Immunogen range: 151-250/355.

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