KLB Polyclonal Antibody

Applications

WB
ELISA
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human

Overview
Catalog #	bs-16748R
Product Name	KLB Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human KLB
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	152831
Swiss Prot	Q86Z14
Synonyms	Beta-klotho; BetaKlotho; BKL; KLB; KLOTB_HUMAN; Klotho beta like protein; Klotho beta-like protein.
Background	BetaKlotho is a 1044 amino acid single-pass type III membrane protein that plays a key role in bile acid and cholesterol metabolism by suppressing transcription of CYP7A1 (cholesterol 7-alpha-hydroxylase), the rate-limiting enzyme in bile acid synthesis. Homozygous negative betaKlotho mice showed dramatically elevated bile acid synthesis and secretion, as well as a strong upregulation of CYP7A1 and CYP8B1 and resistance to gallstone formation. FGF19 and FGF21 require direct interaction with betaKlotho for activation, intracellular signaling and gene expression modulation. Both Klotho and betaKlotho consist of two internal repeats similar to family 1 glycosidases. BetaKlotho contains two glycosyl hydrolase 1 regions, however since these regions lack essential glutamic acid residues at specific and crucial locations, the domains appear to be inactive.
Application Dilution
WB	IHC-P
ELISA	IHC-F1:100-500
IHC-P	IFICC1:50-200
IHC-F	IF
IF
ICC/IF