STAT1 (Tyr701) Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with , Unconjugated (bs-1657R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with , Unconjugated (bs-1657R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Applications

ELISA
FCM
IHC-P

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Pig
Rabbit

Overview
Catalog #	bs-1657R
Product Name	STAT1 (Tyr701) Polyclonal Antibody
Applications	ELISA, FCM, IHC-P
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Pig, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human STAT1 around the phosphorylation site of tyrosine 701
Modification Site	Tyr701
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6772
Subcellular location	Cytoplasm, Nucleus
Synonyms	STAT1Tyr701; signal transducers and activators of transcription 1; DKFZp686B04100; ISGF 3; Phosphorylated Stat1pTyr701; Signal transducer and activator of transcription 1 91kDa; Signal transducer and activator of transcription 1 alpha/beta; STAT 1; STAT 91; STAT91; Transcription factor ISGF 3 components p91 p84.
Background	The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq].
Application Dilution
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400

Applications

Reactivity

Predicted Reactivity

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