Help

HEY1 polyclonal antibody

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Horse
Overview
Catalog # bs-16500R-100uL
Product Name HEY1 polyclonal antibody
Applications
WB ELISA IHC-P IHC-F IF
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Sheep, Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Immunogen Range 51-150/304
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 23462
Swiss Prot Q9Y5J3
Subcellular location Nucleus
Background

This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.

Application Dilution
WB
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF 1:100-500

Frequently asked questions

Which applications is HEY1 polyclonal antibody (bs-16500R) validated for, and at what dilution?

bs-16500R is validated for WB; ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF (1:100-500); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-16500R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Free sample offer

Try a recombinant sample

20 µL sample

Freewith your paid order

View product details (opens in a new tab)

View cart

US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

Primary antibody added to your cart

Need a secondary antibody?

Product validation image

Confirm application and cross-reactivity before use. Prices shown are before eligible cart discounts. Shipping and tax are calculated at checkout.

View cart