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CaMK2 (Thr287) Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-CaMKII (Thr286) Polyclonal Antibody, Unconjugated (bs-1647R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-CaMKII (Thr286) Polyclonal Antibody, Unconjugated (bs-1647R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Phospho-CaMKII (Thr286) Polyclonal Antibody, Unconjugated (bs-1647R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Phospho-CaMKII (Thr286) Polyclonal Antibody, Unconjugated (bs-1647R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
L1 and L2 rat brain lysates probed with Anti Phospho-CaMKII (Thr286) Polyclonal Antibody, Unconjugated (bs-1647R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 54kD. Observed band size: 54kD
L1 and L2 rat brain lysates probed with Anti Phospho-CaMKII (Thr286) Polyclonal Antibody, Unconjugated (bs-1647R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 54kD. Observed band size: 54kD
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (bs-1647R) at 1:400 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (bs-1647R) at 1:400 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (bs-1647R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (bs-1647R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum)); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (bs-1647R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum)); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (bs-1647R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

  • WB
  • ELISA
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Sheep
  • Chicken
  • Rabbit
Overview
Catalog # bs-1647R
Product Name CaMK2 (Thr287) Polyclonal Antibody
Applications WB, ELISA, IHC-P
Reactivity Human, Mouse, Rat
Predicted Reactivity Sheep, Chicken, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from mouse CaMK2 alpha around the phosphorylation site of Thr287
Modification Site Thr287
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 12322
Swiss Prot P11798
Subcellular location Cell membrane
Synonyms CaMKII; R74975; mKIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; Camk2a
Background CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity (By similarity).
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400

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