VEGF-C Polyclonal Antibody

Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Kidney lysates; Lane 3: Rat Thymus lysates; Lane 4: Rat Lymph node lysates; Lane 5: Rat Kidney lysates probed with VEGF-C Polyclonal Antibody, Unconjugated (bs-1586R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with VEGF-C Polyclonal Antibody(bs-1586R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).