GLP-1R Polyclonal Antibody

Name: GLP-1R Polyclonal Antibody
Brand: Bioss
SKU: bs-1559R
Price: 112.5 USD
Availability: InStock

$Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$Mouse liver lysates probed with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37\u02daC.$

Mouse liver lysates probed with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37\u02daC.

$Lane 1: Rat Cerebrum lysates probed with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Rat Cerebrum lysates probed with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

U-937 cells were stained with GLP-1R Polyclonal Antibody(bs-1559R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

$Lane 1: Mouse Pancreas lysates; Lane 2: Mouse Lung lysates probed with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Pancreas lysates; Lane 2: Mouse Lung lysates probed with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Lane 1: Mouse Pancreas tissue lysates; Lane 2: Mouse Liver tissue lysates( negative control) probed with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.$

Lane 1: Mouse Pancreas tissue lysates; Lane 2: Mouse Liver tissue lysates( negative control) probed with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-1559r-20uL
Product Name	GLP-1R Polyclonal Antibody
Applications	WB IHC-P IHC-F IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from rat GLP-1R
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2740
Swiss Prot	P32301
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Glucagon like peptide-1 receptor; GLP 1 R; GLP 1 receptor; GLP 1R; Glp1r; GLP; glp1r; Glucagon like peptide 1 receptor; RATGL1RCP; Glip; MGC138331; GLP-1-R; GLP1R_HUMAN; Glucagon-like peptide 1 receptor; GLP-1 receptor; GLP-1-R; GLP-1R; Precursor.
Application Dilution
WB	=1:500-2000
IHC-P	IHC-P=1:200-1000
IHC-F	IHC-F=1:200-1000
IF	IF=1:200-1000

Frequently asked questions

Which applications is GLP-1R Polyclonal Antibody (bs-1559R) validated for, and at what dilution?

bs-1559R is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:200-1000); IHC-F (IHC-F=1:200-1000); IF (IF=1:200-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GLP-1R Polyclonal Antibody (bs-1559R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-1559R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GLP-1R Polyclonal Antibody (bs-1559R)?

KLH conjugated synthetic peptide derived from rat GLP-1R

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GLP-1R Polyclonal Antibody

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Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

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