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Bim Polyclonal Antibody

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-1488R-100uL
Product Name Bim Polyclonal Antibody
Applications
WB ELISA FCM IHC-P
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human of human Bim
Immunogen Range 51-150/198
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 10018
Swiss Prot O43521
Subcellular location Cytoplasm, Cell membrane
Synonyms BAM; BIM; BOD; Bcl-2-like protein 11; Bcl2-L-11; Bcl2-interacting mediator of cell death; BCL2L11
Background Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Frequently asked questions

Which applications is Bim Polyclonal Antibody (bs-1488R) validated for, and at what dilution?

bs-1488R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Bim Polyclonal Antibody (bs-1488R) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Pig, Horse.

How should bs-1488R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Bim Polyclonal Antibody (bs-1488R)?

KLH conjugated synthetic peptide derived from human of human Bim Immunogen range: 51-150/198.

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