CD177 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-1482R-100uL |
| Product Name | CD177 Polyclonal Antibody |
| Applications |
WB
IHC-P
|
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat CD177 |
| Immunogen Range | 521-650/818 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 499099 |
| Swiss Prot | D3ZCH9 |
| Subcellular location | Cell membrane |
| Synonyms | NB1 GP;PRV-1;HNA-2a;NB1 glycoprotein;Neutrophil Glycoprotein NB1; RGD1562941; CD177 antigen; Polycythemia rubra vera protein 1; PRV-1; NB1 glycoprotein; NB1 GP; Human neutrophil alloantigen 2a; HNA-2a; CD177; CD177_HUMAN; CD177_MOUSE. |
| Background | Cd177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97% of the caucasian population. against NB1 can be involved in neonatal alloimmune neutropenia (NAN). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is CD177 Polyclonal Antibody (bs-1482R) validated for, and at what dilution?
bs-1482R is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD177 Polyclonal Antibody (bs-1482R) react with?
Tested reactivity: Mouse, Rat.
How should bs-1482R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD177 Polyclonal Antibody (bs-1482R)?
KLH conjugated synthetic peptide derived from rat CD177 Immunogen range: 521-650/818.