Jagged1 Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse colon labeled with Anti-Jagged1\/CD339 Polyclonal Antibody, Unconjugated (bs-1448R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded mouse colon labeled with Anti-Jagged1\/CD339 Polyclonal Antibody, Unconjugated (bs-1448R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Jagged1) polyclonal Antibody, Unconjugated (bs-1448R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Jagged1) polyclonal Antibody, Unconjugated (bs-1448R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Lane 1: Recombinant human CD339 protein, N-His(bs-42225P) probed with Jagged1 Polyclonal Antibody, Unconjugated (bs-1448R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Recombinant human CD339 protein, N-His(bs-42225P) probed with Jagged1 Polyclonal Antibody, Unconjugated (bs-1448R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-1448R
Product Name	Jagged1 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Jagged1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	182
Synonyms	JAG1; AGS; AHD; AWS; CD339; HJ1; JAGL1;MGC104644; Ser 1; CD 339; CD339; CD339 antigen; Headturner; Htu; Jag 1; Jagged-1; Jagged 1; Jagged 1 Alagille syndrome; JAGL1; Ser-1; Ser1; Serrate-1; Slalom.
Background	The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq].
Application Dilution
WB	1:500-1000
IHC-P	1:100-500
IHC-F	IFIHC-P1:100-500
IF	ICC1:100
ICC/IF