DEPDC1B/BRCC3 Polyclonal Antibody

Formalin-fixed and paraffin embedded human brain glioma labeled with Anti-DEPDC1B Polyclonal Antibody, Unconjugated (bs-14278R) at 1:500 followed by conjugation to the secondary antibody and DAB staining.

$Lane 1: HepG2 cell lysates; Lane 2: A549 cell lysates probed with DEPDC1B\/BRCC3 Polyclonal Antibody, Unconjugated (bs-14278R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.$

Lane 1: HepG2 cell lysates; Lane 2: A549 cell lysates probed with DEPDC1B\/BRCC3 Polyclonal Antibody, Unconjugated (bs-14278R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates probed with DEPDC1B\/BRCC3 Polyclonal Antibody, Unconjugated (bs-14278R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates probed with DEPDC1B\/BRCC3 Polyclonal Antibody, Unconjugated (bs-14278R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Cow
Rabbit

Overview
Catalog #	bs-14278R
Product Name	DEPDC1B/BRCC3 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Cow, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human DEPDC1B
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	55789
Swiss Prot	Q8WUY9
Subcellular location	Cytoplasm
Synonyms	XTP1; BRCC3; DEP domain-containing protein 1B; HBV X-transactivated gene 8 protein; HBV XAg-transactivated protein 8; DEPDC1B; XTP8
Background	GTPase activator activity.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200