Pan Granzyme Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Others

Predicted Reactivity

  • Rat
  • Cow
  • Pig
Overview
Catalog # bs-1351R-Cy5
Product Name Pan Granzyme Polyclonal Antibody, Cy5 Conjugated
Applications WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity Specific towards Granzyme B and Granzyme H
Reactivity Human, Mouse, Others
Predicted Reactivity Rat, Cow, Pig
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GZMB
Immunogen Range 21-130/247
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3002
Swiss Prot P10144
Subcellular location Cytoplasm
Synonyms HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1; Granzyme B; C11; CTLA-1; Cathepsin G-like 1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; T-cell serine protease 1-3E; GZMB; GRB, Granzyme B, Granzyme H
Background This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200