DLGAP4 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-SAPAP4 Polyclonal Antibody, Unconjugated (bs-12140R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining$

Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-SAPAP4 Polyclonal Antibody, Unconjugated (bs-12140R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

$Rat brain lysates probed with SAPAP4\/DLGAP4 Polyclonal Antibody, unconjugated (bs-12140R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.$

Rat brain lysates probed with SAPAP4\/DLGAP4 Polyclonal Antibody, unconjugated (bs-12140R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SAPAP4\/DLGAP4 Polyclonal Antibody, Unconjugated (bs-12140R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 90 minutes and DAPI for nuclei staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SAPAP4\/DLGAP4 Polyclonal Antibody, Unconjugated (bs-12140R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 90 minutes and DAPI for nuclei staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Sheep
Pig
Horse
Rabbit
GuineaPig

Overview
Catalog #	bs-12140R
Product Name	DLGAP4 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, GuineaPig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SAPAP4/DLGAP4
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cell membrane
Synonyms	DAP 4; DAP-4; DAP4; Discs, large Drosophila homolog associated protein 4; Disks large associated protein 4; Disks large-associated protein 4; DLGAP4; DLGP4_HUMAN; DLP4; PSD 95/SAP90 binding protein 4; PSD-95/SAP90-binding protein 4; RP5-977B1.6; SAP90/PSD-95-associated protein 4; SAPAP 4; SAPAP-4; SAPAP4.
Background	A guanylate kinase is a phosphotransferase that produces ADP and GDP from the substrates ATP and GMP. SAPAP4 is a 992 amino acid protein that likely localizes to the postsynaptic membrane of neurons to enhance neuronal signaling. SAPAP4 could act as a signaling molecule which interacts with the human genes DLG1 and DLG4/PSD-95. The gene encoding SAPAP4, DLGAP4, maps to human chromosome 20. Comprising approximately 2% of the human genome, chromosome 20 contains nearly 63 million bases that encode over 600 genes, some of which are associated with Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, spinal muscular atrophy, ring chromosome 20 epilepsy syndrome and Alagille syndrome. Additionally, chromosome 20 contains a region with numerous genes which are thought important for seminal production and may be potential targets for male contraception.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200