ASIC3 Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12132R-100uL |
| Product Name | ASIC3 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Pig, Horse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ASIC3 |
| Immunogen Range | 342-420/531 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane, Extracellular matrix |
| Synonyms | ASICs3; ACCN 3; ACCN3; ASIC3_HUMAN; Acid sensing ion channel 3; Acid-sensing ion channel 3; Amiloride sensitive cation channel 3; Amiloride sensitive cation channel 3 testis; Amiloride-sensitive cation channel 3; ASIC 3; ASIC3; DRASIC; hASIC 3; hASIC3; hTNaC 1; hTNaC1; Modulatory subunit of ASIC 2a; Modulatory subunit of ASIC2a; Neuronal amiloride sensitive cation channel 3; Neuronal amiloride-sensitive cation channel 3; Proton gated cation channel subunit; SLNAC 1; SLNAC1; Testis sodium channel 1; TNaC 1; TNaC1. |
| Background | Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ASIC3 Polyclonal Antibody (bs-12132R) validated for, and at what dilution?
bs-12132R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12132R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ASIC3 Polyclonal Antibody (bs-12132R)?
KLH conjugated synthetic peptide derived from human ASIC3 Immunogen range: 342-420/531.