ACCN2 Polyclonal Antibody

$Lane 1: U87mg Cell lysates; Lane 2: BV2 Cell lysates; probed with ACCN2 Polyclonal Antibody, unconjugated (bs-12128R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: U87mg Cell lysates; Lane 2: BV2 Cell lysates; probed with ACCN2 Polyclonal Antibody, unconjugated (bs-12128R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ACCN2 Polyclonal Antibody, Unconjugated (bs-12128R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ACCN2 Polyclonal Antibody, Unconjugated (bs-12128R) at 1:400 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
IF(IHC-P)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-12128R
Product Name	ACCN2 Polyclonal Antibody
Applications	WB, ELISA, IF(IHC-P)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ACCN2/ASIC1
Immunogen Range	332-460/528
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cell membrane
Synonyms	ASIC1; ACCN2; ACCN2_HUMAN; Acid sensing ion channel 1; Acid sensing ion channel 1a protein; Acid sensing ion channel; Acid-sensing ion channel 1; AI843610; Amiloride sensitive cation channel 2 neuronal; Amiloride-sensitive cation channel 2; ASIC; ASIC1; ASIC1A; B530003N02Rik; BNAC2; Brain sodium channel 2; Cation channel amiloride sensitive neuronal 2; hBNaC2; neuronal.
Background	Acid sensing ion channel ASIC1 is present in brain as a 4.3-kb transcript with localization to rat dorsal root ganglia. In situ hybridization of rat brain suggests that ASIC1 is most abundant in the main olfactory bulb, cerebral cortex, hippocampal formation, habenula, basolateral amygdaloid nuclei and cerebellum. ASIC1 and H+-gated currents may contribute to the development of fear and anxiety. ASIC2, also designated amiloride-sensitive cation channel 1, neuronal (ACCN1), mammalian degenerin, BNAC1 (MDEG) and brain Na+ channel 1, mediates the normal detection of light touch. ASIC2 mRNA is abundant in brain, specifically in neurons. ASIC2 is expressed as 2.7- and 3.7-kb transcripts in brain and spinal cord tissues. ASIC3, also designated SLNAC1 and TNaC1, mediates detection of lasting pH changes and is involved in modulating moderate- to high-intensity pain sensation. ASIC4, also designated ACCN4 and BNAC4, is abundant in pituitary gland and is also present in the inner ear.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IF(IHC-P)	1:50-200

Applications

Reactivity

Predicted Reactivity

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