CTLA-4/CD152 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse colon labeled with Rabbit Anti CTLA-4 Polyclonal Antibody, Unconjugated (bs-1179R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin-embedded mouse tumor labeled with Anti-CTLA-4 Polyclonal Antibody, Unconjugated(bs-1179R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.DAPI was used to stain the cell nucleus.$

Formalin-fixed and paraffin-embedded mouse tumor labeled with Anti-CTLA-4 Polyclonal Antibody, Unconjugated(bs-1179R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.DAPI was used to stain the cell nucleus.

$Formalin-fixed and paraffin-embedded human liver cancer labeled with Anti-CTLA-4 Polyclonal Antibody, Unconjugated(bs-1179R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.DAPI was used to stain the cell nucleus.$

Formalin-fixed and paraffin-embedded human liver cancer labeled with Anti-CTLA-4 Polyclonal Antibody, Unconjugated(bs-1179R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.DAPI was used to stain the cell nucleus.

$Lane 1: Mouse Lung tissue lysates; Lane 2: Rat Spleen tissue lysates; Lane 3: Rat Lung tissue lysates probed with CTLA4 Polyclonal Antibody, Unconjugated (bs-1179R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Lung tissue lysates; Lane 2: Rat Spleen tissue lysates; Lane 3: Rat Lung tissue lysates probed with CTLA4 Polyclonal Antibody, Unconjugated (bs-1179R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Thymus tissue lysates; Lane 3: Mouse Lymph node tissue lysates; Lane 4: Rat Spleen tissue lysates; Lane 5: Rat Thymus tissue lysates; Lane 6: Rat Lymph node tissue lysates; Lane 7: Human Raji cell lysates; Lane 8: Human K562 cell lysates probed with CTLA4 Polyclonal Antibody, Unconjugated (bs-1179R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.$

Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Thymus tissue lysates; Lane 3: Mouse Lymph node tissue lysates; Lane 4: Rat Spleen tissue lysates; Lane 5: Rat Thymus tissue lysates; Lane 6: Rat Lymph node tissue lysates; Lane 7: Human Raji cell lysates; Lane 8: Human K562 cell lysates probed with CTLA4 Polyclonal Antibody, Unconjugated (bs-1179R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-1179r
Product Name	CTLA-4/CD152 Polyclonal Antibody
Applications	WB IHC-P IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CTLA-4
Immunogen Range	183-223/223
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1493
Subcellular location	Cytoplasm
Synonyms	Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CTLA4; CD152
Background	Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200