N-cadherin Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-CDH2\/N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CDH2\/N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Formalin-fixed and paraffin-embedded rat brain labeled with Anti-CDH2\/N-cadherin Polyclonal Antibody, Unconjugated(bs-1172R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.$

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-CDH2\/N-cadherin Polyclonal Antibody, Unconjugated(bs-1172R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Mouse breast cancer lysates probed with Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.

$Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining$

Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-1172R
Product Name	N-cadherin Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human N-cadherin
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1000
Swiss Prot	P19022
Subcellular location	Cell membrane
Synonyms	CDHN; NCAD; CD325; CDw325; Cadherin-2; Neural cadherin; N-cadherin; CDH2; CADH2_HUMAN
Background	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200