NPW/Neuropeptide W Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11531R-100uL |
| Product Name | NPW/Neuropeptide W Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Neuropeptide W |
| Immunogen Range | 33-55/165 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Subcellular location | Secreted |
| Synonyms | hPPL8; L8; L8C; Neuropeptide W; PPL8; PPNPW; Preproprotein L8; NPW_HUMAN. |
| Background | NPW is a 165 amino acid secreted protein that is cleaved into two chains: neuropeptide W-23 (also designated NPW23 or L8) and neuropeptide W-30 (also known as NPW30 or L8C). Both NPW neuropeptides activate G-protein coupled receptors in the central nervous system to enhance cortisol secretion. Highly expressed in lymphoblastic leukemia, colorectal adenocarcinoma, fetal kidney, trachea and substantia nigra, NPW is also found at low levels in placenta, ovary, testis and uterus. NPW functions in organization of neuroendocrine signals and is also thought to enhance food and water intake as well as stress responses. The gene encoding NPW maps to human chromosome 16p13.3. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NPW/Neuropeptide W Polyclonal Antibody (bs-11531R) validated for, and at what dilution?
bs-11531R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NPW/Neuropeptide W Polyclonal Antibody (bs-11531R) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow.
How should bs-11531R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NPW/Neuropeptide W Polyclonal Antibody (bs-11531R)?
KLH conjugated synthetic peptide derived from human Neuropeptide W Immunogen range: 33-55/165.