SRC Polyclonal Antibody

Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.", "Category"=>"Primary Antibody", "Clonality"=>"Polyclonal", "Concentration"=>"1ug/ul", "Conjugation"=>"Unconjugated", "Cross Reactive Species"=>"Human, Mouse, Rat", "Datasheet URL"=>"https://www.biossusa.com/store/datasheets/bs-1135R", "Gene ID"=>"6714", "Gene ID Link"=>"Entrez Gene: 6714", "Host"=>"Rabbit", "images"=>"[{\"id\":\"15926\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"1\",\"origin_notes\":\"\",\"product_id\":\"2139\",\"productconjugation_id\":null,\"application_id\":\"4\",\"application_note\":\"\",\"dilution\":\"1:400\",\"cross_reactive_species_id\":\"3\",\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\\u00b0C for 20min; Antibody incubation with SRC Polyclonal Antibody, Unconjugated (bs-1135R) at 1:400 overnight at 4\\u00b0C, followed by a conjugated secondary and DAB staining.\",\"sample_type_id\":\"2\",\"sample_details\":null,\"rating\":\"3\",\"disease_id\":null,\"sample_tissue_id\":\"2\",\"cell_line_id\":null,\"protocols_used\":\"Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\\u00b0C for 30min; Antibody incubation with (SRC) Polyclonal Antibody, Unconjugated (bs-1135R) at 1:400 overnight at 4\\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.\",\"additional_notes\":\"\",\"filepath_raw\":\"2139\\/15926_raw.jpg\",\"filepath_med\":\"2139\\/15926_med.jpg\",\"filepath_thumb\":\"2139\\/15926_thumb.jpg\",\"display_order\":\"1\",\"lock_version\":\"1\",\"created_at\":\"2017-04-17 17:35:30\",\"updated_at\":\"2017-04-17 17:35:31\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/15926\"},{\"id\":\"15927\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"1\",\"origin_notes\":\"\",\"product_id\":\"2139\",\"productconjugation_id\":null,\"application_id\":\"4\",\"application_note\":\"\",\"dilution\":\"1:400\",\"cross_reactive_species_id\":\"2\",\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\\u00b0C for 20min; Antibody incubation with SRC Polyclonal Antibody, Unconjugated (bs-1135R) at 1:400 overnight at 4\\u00b0C, followed by a conjugated secondary and DAB staining.\",\"sample_type_id\":\"2\",\"sample_details\":null,\"rating\":\"2\",\"disease_id\":null,\"sample_tissue_id\":\"20\",\"cell_line_id\":null,\"protocols_used\":\"Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\\u00b0C for 20min; Antibody incubation with SRC Polyclonal Antibody, Unconjugated (bs-1135R) at 1:400 overnight at 4\\u00b0C, followed by a conjugated secondary and DAB staining.\",\"additional_notes\":\"\",\"filepath_raw\":\"2139\\/15927_raw.jpg\",\"filepath_med\":\"2139\\/15927_med.jpg\",\"filepath_thumb\":\"2139\\/15927_thumb.jpg\",\"display_order\":\"2\",\"lock_version\":\"1\",\"created_at\":\"2017-04-17 17:36:21\",\"updated_at\":\"2017-04-17 17:36:21\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/15927\"}]", "Immunogen Sequence"=>"2-100/541", "Isotype"=>"IgG", "Lead Time"=>"3 to 5 business days.", "Modification"=>"Unmodified", "Predicted Reactivity"=>"Dog, Cow", "Product URL"=>"https://www.biossusa.com/products/bs-1135R", "Purification"=>"Purified by Protein A.", "Size"=>"100ul", "Source"=>"KLH conjugated synthetic peptide derived from human SRC", "Storage"=>"0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.", "Storage Buffer"=>"0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.", "Storage Condition"=>"Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.", "Subcellular Locations"=>"Cytoplasm, Nucleus, Cell membrane", "Swiss Prot"=>"P12931", "Swiss Prot Link"=>"Swiss Prot: P12931", "Synonyms"=>"ASV; Avian sarcoma virus; c SRC; CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA; cSrc; EC 2.7.10.2; Neuronal CSRC tyrosine specic protein kinase; Neuronal SRC; Oncogene SRC; OTTHUMP00000030931; OTTHUMP00000174476; OTTHUMP00000174477; p60 Src; p60-Src; p60Src; pp60c src; pp60c-src; pp60csrc; Proto oncogene tyrosine protein kinase Src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; Protooncogene SRC; protooncogene SRC Rous sarcoma; Src; SRC Oncogene; SRC_MOUSE; SRC1; Tyrosine kinase pp60c src; Tyrosine protein kinase SRC 1; Tyrosine protein kinase SRC1; v src avian sarcoma Schmidt Ruppin A2 viral oncogene homolog; V src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian; v src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian; vsrc avian sarcoma Schmidt Ruppin A2 viral oncogene homolog; SRC_HUMAN.", "Target Protein"=>"SRC"} -->