VGLUT1/ VGLUT2 Polyclonal Antibody

$Antigen: bs-11167P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-11167P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

Mouse brain lysates probed with Rabbit Anti-VGLUT1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody for 90 min at 37˚C.

$Lane 1: Rat Cerebrum tissue lysates; Lane 2: Human SH-SY5Y cell lysates; Lane 3: Human MCF-7 cell lysates probed with VGLUT1\/BNP1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Rat Cerebrum tissue lysates; Lane 2: Human SH-SY5Y cell lysates; Lane 3: Human MCF-7 cell lysates probed with VGLUT1\/BNP1 Polyclonal Antibody, Unconjugated (bs-11167R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-11167R
Product Name	VGLUT1/ VGLUT2 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Specificity	The immunogen shares 100% sequence similarity with VGLUT2.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human VGLUT1
Immunogen Range	301-400/560
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	57030
Synonyms	BNPI; Brain specic Na + dependent inorganic phosphate cotransporter; Brain specic Na dependent inorganic phosphate cotransporter; Brain-specic Na+-dependent inorganic phosphate cotransporter; Slc17a7; Solute carrier family 17 sodium-dependent inorganic phosphate cotransporter, member 7; Solute carrier family 17 member 7; Vesicular glutamate transporter 1; VGLU1_HUMAN; VGluT1; VGLU2_HUMAN; VGlut2; Vesicular glutamate transporter 2.
Background	Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Involved in the regulation of retinal hyaloid vessel regression during postnatal development.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200

Applications

Reactivity

Search our catalog of hundreds of thousands of products