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glypican 3 Polyclonal Antibody

Applications

  • WB
  • ELISA
  • FCM

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-1112R-100uL
Product Name glypican 3 Polyclonal Antibody
Applications
WB ELISA FCM
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human glypican 3
Immunogen Range 201-300/580
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2719
Subcellular location Secreted, Cell membrane, Extracellular matrix
Synonyms glypican 3; glypican3; glypican-3; GPC3; Intestinal protein OCI-5; GTR2-2; MXR7.Intestinal protein OCI-5.
Background Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome. (Also known as glypican 3; Intestinal protein OCI-5; GTR2-2; MXR7; Glypican-3 precursor).
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100

Frequently asked questions

Which applications is glypican 3 Polyclonal Antibody (bs-1112R) validated for, and at what dilution?

bs-1112R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does glypican 3 Polyclonal Antibody (bs-1112R) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bs-1112R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise glypican 3 Polyclonal Antibody (bs-1112R)?

KLH conjugated synthetic peptide derived from human glypican 3 Immunogen range: 201-300/580.

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