MPO Polyclonal Antibody

HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MPO Polyclonal Antibody(bs-1061R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

$HL60 cell; 4% Paraformaldehyde-fixed; Ice-cold methanol at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Anti-Myeloperoxidase) polyclonal antibody, A555 conjugated (bs-1061R-A555) 1:50, 90 minutes at 37\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.$

HL60 cell; 4% Paraformaldehyde-fixed; Ice-cold methanol at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Anti-Myeloperoxidase) polyclonal antibody, A555 conjugated (bs-1061R-A555) 1:50, 90 minutes at 37\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Horse
Rabbit
GuineaPig

Overview
Catalog #	bs-1061R
Product Name	MPO Polyclonal Antibody
Applications	WB IHC-P IF(IHC-P)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Horse, Rabbit, GuineaPig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MPO
Immunogen Range	50-100/745
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4353
Swiss Prot	P05164
Subcellular location	Lysosome
Synonyms	Myeloperoxidase; MPO
Background	Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200