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MIF Polyclonal Antibody

Formalin-fixed and paraffin embedded rat skin labeled with Anti-MIF Polyclonal Antibody, Unconjugated (bs-1044R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat skin labeled with Anti-MIF Polyclonal Antibody, Unconjugated (bs-1044R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human glioma labeled with Anti-MIF Polyclonal Antibody, Unconjugated (bs-1044R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human glioma labeled with Anti-MIF Polyclonal Antibody, Unconjugated (bs-1044R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Rat Aldrenal gland Lysates; Lane 2: Human HL-60 cell Lysates. Probed with MIF polyclonal Antibody, unconjugated (bs-1044R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Aldrenal gland Lysates; Lane 2: Human HL-60 cell Lysates. Probed with MIF polyclonal Antibody, unconjugated (bs-1044R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Pig
Overview
Catalog # bs-1044R
Product Name MIF Polyclonal Antibody
Applications WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Pig
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MIF
Immunogen Range 51-115/115
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4282
Swiss Prot P14174
Subcellular location Cytoplasm, Secreted
Synonyms GIF; GLIF; MMIF; Macrophage migration inhibitory factor; MIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase
Background Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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