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DSPP Polyclonal Antibody

Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Rabbit Anti-DSPP Polyclonal Antibody, Unconjugated (bs-10316R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Rabbit Anti-DSPP Polyclonal Antibody, Unconjugated (bs-10316R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: EC-9706 cell lysates; Lane 2: MCF-7 cell lysates probed with DSPP Polyclonal Antibody, Unconjugated (bs-10316R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: EC-9706 cell lysates; Lane 2: MCF-7 cell lysates probed with DSPP Polyclonal Antibody, Unconjugated (bs-10316R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human DU145 cell lysates probed with DSPP Polyclonal Antibody, Unconjugated (bs-10316R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human DU145 cell lysates probed with DSPP Polyclonal Antibody, Unconjugated (bs-10316R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bs-10316R
Product Name DSPP Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DSPP
Clonality Polyclonal
Clone # #REF!
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1834
Swiss Prot Q9NZW4
Subcellular location Extracellular matrix
Synonyms DPP; DSP; DGI1; DMP3; DTDP2; DFNA39; Dentin sialophosphoprotein; DSPP
Background DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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